A single mutation in the DSL motif of the acyl carrier protein can prevent its in vivo modification by E. coli Holo-acyl carrier protein synthase (AcpS)
Chetna Dhembla, Debodyuti Sadhukhan, Rashima Prem, Shivangi Vaish, Shalini Verma, Suman Kundu, Monica Sundd
The paper identifies a single mutation (D35N) in the DSL motif of acyl carrier proteins (ACP) that prevents in vivo modification by E. coli AcpS, enabling the expression of apo-ACP and demonstrating its applicability across different ACPs.
bioRxiv · Jan 28, 2025 · PreprintBiochemistry