Comparison of a long-read amplicon sequencing approach to short-read amplicons for microbiome analysis
Brandon O’Sullivan, Katherine W. Herbst, Alexander H. Hogan, Michele A. Maltz, Justin David Radolf, David Lawrence, Michael A. Lynes, Juan Carlos Salazar, Joerg Graf, The Connecticut Children’s COVID Collaborative, Michael Brimacombe, Christopher L. Carroll, Kyle J. Carson, Taylor R. T. Dagenais, Catalina De La Cruz Macias, , Surender Khurana
This paper compares StrainID long-read amplicon sequencing to short-read amplicons for microbiome analysis, showing similar performance overall but better phylogenetic diversity and taxonomic classification in saliva samples.
Microbiology Spectrum · May 19, 2026Peer-reviewedMicrobiology