Split inteins for generating combinatorial non-ribosomal peptide libraries
Patrick Gonschorek, Christopher S. Wilson, Christian Schelhas, Kenan A. J. Bozhüyük, Peter Grün, Helge B. Bode
This paper introduces split inteins to enable modular NRPS engineering, generating 324 hybrid NRPS and over 200 novel peptides through combinatorial transformation.
bioRxiv · Oct 2, 2025 · PreprintSynthetic Biology