Peter Grün

Site-Specific Introduction of Non-Canonical Amino Acids into natural and engineered Non-Ribosomal Peptides

The paper introduces non-canonical amino acids into non-ribosomal peptides by engineering NRPS systems and performing post-enzymatic chemical synthesis in bacterial culture extracts.

Split inteins for generating combinatorial non-ribosomal peptide libraries

This paper introduces split inteins to enable modular NRPS engineering, generating 324 hybrid NRPS and over 200 novel peptides through combinatorial transformation.

Exploiting the inherent promiscuity of the acyl transferase of the stambomycin polyketide synthase for the mutasynthesis of analogues

This paper uses mutasynthesis to generate stambomycin derivatives by engineering a bacterial strain and leveraging a promiscuous acyl transferase domain, resulting in new analogues with similar or reduced bioactivity.

An Unconventional Melanin Biosynthetic Pathway in Ustilago maydis

This paper identifies a novel melanin biosynthetic pathway in Ustilago maydis involving multiple polyketide synthases and other enzymes, distinct from known fungal melanin pathways.

Modification and de novo design of non-ribosomal peptide synthetases (NRPS) using specific assembly points within condensation domains

This paper describes a method to modify and design non-ribosomal peptide synthetases (NRPSs) using a new fusion point in condensation domains to produce peptides, including non-natural amino acids, at high yields.