Arrayed single-gene perturbations identify drivers of human anterior neural tube closure
Roya E. Huang, Giridhar M. Anand, Heitor C. Megale, Jason Chen, Chudi Abraham-Igwe, Sharad Ramanathan
The paper develops a platform for high-throughput CRISPRi screening in hPSC-derived organoids to identify genetic drivers of human anterior neural tube closure, finding ZIC2, SOX11, and ZNF521 as key regulators.
eLife · Jul 7, 2026Peer-reviewedDevelopmental Biology