Deep oncopanel sequencing reveals fixation time- and within block position-dependent quality degradation in FFPE processed samples
Y. Zhang, T. M. Blomquist, R. Kusko, D. Stetson, Z. Zhang, L. Yin, R. Sebra, B. Gong, J. S. LoCoco, V. K. Mittal, N. Novoradovskaya, J.-Y. Yeo, N. Dominiak, J. Hipp, A. Raymond, , J. Xu
This study identifies FFPE processing errors in oncology panel sequencing by analyzing variants from technical artifacts in FFPE samples with varying fixation times and block positions.
bioRxiv · Apr 6, 2021 · PreprintCancer Biology