The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity
K. J. Forsberg, D. T. Schmidtke, R. Werther, R. V. Uribe, D. Hausman, M. O. A. Sommer, B. L. Stoddard, B. K. Kaiser, H. S. Malik
The paper identifies AcrIIA22 as an anti-CRISPR protein that inhibits SpyCas9 by relieving DNA torsion in plasmids.
bioRxiv · Sep 28, 2020 · PreprintMicrobiology