Advancing Understanding of DNA-BfiI Restriction Endonuclease Cis and Trans Interactions through smFRET Technology
M. Tutkus, S. Ivanovaite, J. Paksaite, A. Kopustas, G. Karzaite, D. Rutkauskas, A. Silanskas, G. Sasnauskas, M. Zaremba, S. K. Jones
This paper develops smFRET assays to study BfiI-DNA interactions, showing longer binding durations on target sites and minimal conformational changes.
bioRxiv · Mar 31, 2023 · PreprintBiophysics