Anionic polymers stabilize Cas9 ribonucleoprotein nanoparticles to improve direct protein delivery and genome editing efficiencies in plant protoplasts
Joshua D. Hubbard, Sophia Tomatz, Nicole Carll, Juliana L. Matos, Danya Hassan, T. Ly, Markita Patricia Landry
This paper shows that adding anionic polymers like polyglutamic acid to protoplast transfection protocols improves CRISPR-Cas9 genome editing efficiency in plants.
bioRxiv · Feb 2, 2025 · PreprintPlant Biology