T. Ly

Offsetting ROS-mediated arrest of endothelial fenestration dynamics permits long-term optical super-resolution- and AFM-imaging

The paper identifies ROS as a cause of fenestration arrest in LSECs during imaging and proposes a strategy to preserve dynamics for long-term super-resolution and AFM imaging.

Anionic polymers stabilize Cas9 ribonucleoprotein nanoparticles to improve direct protein delivery and genome editing efficiencies in plant protoplasts

This paper shows that adding anionic polymers like polyglutamic acid to protoplast transfection protocols improves CRISPR-Cas9 genome editing efficiency in plants.

A chemical-genetic system to rapidly inhibit the PP2A-B56 phosphatase

The paper develops a chemical-genetic system to inhibit PP2A-B56 phosphatase by blocking SLiM-binding pockets, revealing its role in kinetochore-microtubule attachments.

Rewiring of the phosphoproteome executes two meiotic divisions

The paper identifies how Spo13Meikin rewires the phosphoproteome through kinase activity to drive two meiotic divisions in budding yeast.

Cell overgrowth during G1 arrest triggers an osmotic stress response and chronic p38 activation to promote cell cycle exit

The paper shows that CDK4/6 inhibitor-induced G1 arrest causes cell overgrowth, triggering osmotic stress and p38-p53-p21 signaling to promote cell cycle exit.

Cyclin A and Cks1 promote kinase consensus switching to non-proline directed CDK1 phosphorylation

The paper shows Cyclin A and Cks1 enable CDK1 to phosphorylate non-proline directed sites, particularly with a +3 lysine residue, altering substrate choice based on CDK1 activity.

Hypoxia shapes the immune landscape in lung injury promoting inflammation persistence

This study shows hypoxia suppresses type I interferon signaling in ALI, leading to monocytopenia and unchecked inflammation, which can be mitigated by CSF1 administration.

Enteroendocrine cell types that drive food reward and aversion

The paper identifies enteroendocrine cell subtypes that influence food reward and aversion through distinct signaling pathways in mice.

CDK4/6 inhibitors induce replication stress to cause long-term cell cycle withdrawal

This study shows CDK4/6 inhibitors cause long-term cell cycle withdrawal by inducing replication stress and p53-dependent effects, linking them to genotoxic stress and potential combination therapies.

Low cell number proteomic analysis using in-cell protease digests reveals a robust signature for cell cycle state classification

This paper introduces an in-cell protease digest method for low-cell-number proteomics and identifies a cell cycle signature for classification.

Cyclin A triggers Mitosis either via Greatwall or Cyclin B

This study shows Cyclin A can trigger mitosis through Greatwall or Cyclin B by regulating Cdk1 activity and phosphorylation of mitotic substrates.

Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS

The paper uses PRIMMUS to analyze proteomic changes during cell cycle progression, identifying phosphorylation sites and their roles in mitotic progression.

Characterisation Of The Biflavonoid Hinokiflavone As A Pre-mRNA Splicing Modulator That Inhibits SENP

This study identifies hinokiflavone as a splicing inhibitor that modulates alternative splicing by inhibiting SUMO protease SENP1 and altering SUMOylation of splicing factors.