Predictive genome-wide CRISPR-mediated gene downregulation for enhanced bioproduction
Ian Sofian Yunus, David N. Carruthers, Yan Chen, Jennifer W. Gin, Edward E. K. Baidoo, Christopher J. Petzold, Héctor García Martín, Paul D. Adams, Aindrila Mukhopadhyay, Taek Soon Lee
This study introduces two methods, FluxRETAP and VAMMPIRE, to improve CRISPRi-mediated gene downregulation for bioproduction by enhancing target identification and multiplexed construct assembly.
bioRxiv · Apr 25, 2025 · PreprintSynthetic Biology