Multiplex single-molecule kinetics of nanopore-coupled polymerases
Mirkó Palla, Sukanya Punthambaker, Benjamin Stranges, Frédéric Vigneault, Jeff Nivala, Daniel J. Wiegand, Aruna Ayer, Timothy K. Craig, Dmitriy E. Gremyachinskiy, Helen Franklin, Shaw Sun, James Pollard, Andrew Trans, Cleoma Arnold, Charles Schwab, , George McDonald Church
The paper demonstrates a single-molecule method to screen polymerase variants using nanopore sequencing and barcoding, enabling multiplexed analysis of kinetic properties.
ACS Nano · Dec 28, 2020Peer-reviewedSynthetic Biology