Identification and Characterization of a Residual Host Cell Protein Hexosaminidase B Associated with N-glycan Degradation During the Stability Study of a Therapeutic Recombinant Monoclonal Antibody Product
X. Li, Y. An, J. Liao, L. Xiao, M. Swanson, K. Martinez-Fonts, J. A. Pavon, E. C. Sherer, V. Jawa, X. Gao, S. Letarte, D. D. Richardson
The paper identifies Hexosaminidase B as a residual host cell protein causing N-glycan degradation in a monoclonal antibody under stability conditions and develops strategies to mitigate its impact.
bioRxiv · Sep 17, 2020Biochemistry