Unbiased and UMI-informed sequencing of cell-free miRNAs at single-nucleotide resolution
Monique A.J. van Eijndhoven, Ernesto Aparicio‐Puerta, Cristina Gómez‐Martín, José M. Medina, Esther E.E. Drees, E.J. Bradley, Leontien Bosch, Chantal Scheepbouwer, Michael Hackenberg, Dirk Michiel Pegtel
The paper introduces IsoSeek, a method for unbiased and UMI-informed sequencing of cell-free miRNAs, revealing extensive uridylation of disease-associated miRNAs and improving isomiR detection in extracellular vesicles.
bioRxiv · May 4, 2021 · PreprintGenomics