Dirk Michiel Pegtel

Ouro-seq: Improved Recovery of Full-Length circRNAs from Samples with Limited RNA Content

Ouro-seq improves full-length circRNA recovery from low-RNA samples, revealing distinct circRNA types and functions in clinical contexts.

Full-length tRNAs lacking a functional CCA tail are selectively sorted into the lumen of extracellular vesicles

The paper identifies that full-length tRNAs with truncated CCA tails are selectively sorted into extracellular vesicles, suggesting a new mechanism for EV cargo selection.

Circulating extracellular vesicles in lung cancer patients are not enriched in tumor-derived DNA fragments as revealed by whole genome sequencing.

The paper shows that EV-DNA in lung cancer patients is not enriched in tumor signal compared to plasma cfDNA, with similar fragmentation and tumor content.

The effect of pre-analytical and physiological variables on cell-free DNA fragmentation.

This study examines how pre-analytical and physiological factors affect cfDNA fragmentation patterns and fragment-end sequences using whole genome sequencing.

Unbiased and UMI-informed sequencing of cell-free miRNAs at single-nucleotide resolution

The paper introduces IsoSeek, a method for unbiased and UMI-informed sequencing of cell-free miRNAs, revealing extensive uridylation of disease-associated miRNAs and improving isomiR detection in extracellular vesicles.