Cutting Through the Artifacts: Dissecting gRNA Impurities with FUSS-seq
Antonino Montalbano, Huan Qiu, Lakshmi Karthik, Sawyer Letourneau, Jianxin Hu, Tirtha Chakraborty, Huanying Gary Ge, John R. Lydeard, Ruijia Wang, Eric Gunnar Anderson
The paper identifies sequencing artifacts in gRNA purity assessment and introduces FUSS-seq to reduce them, improving accuracy for CRISPR-based therapeutics.
bioRxiv · Sep 13, 2025 · PreprintMolecular Biology