Empirical optimization of dual-sgRNA design for in vivo CRISPR/Cas9-mediated exon deletion in mice
Sung-Yeon Lee, Seongwon Ma, Sangjun Davie Jeon, Hyoju Kim, Beomjoon Jo, Seung-Hoon Han, Eunsoo Jang, Jimin Lee, Yong-Kyu Lee, Dasom Lee
This study develops an in vivo-trained model to predict dual-sgRNA efficiency for exon deletion in mice, achieving an AUC of 0.759.
Computational and Structural Biotechnology Journal · Jan 1, 2026Peer-reviewedBioinformatics