Saturated Transposon Analysis in Yeast as a One-step Method to Quantify the Fitness Effects of Gene Disruptions on a Genome-Wide Scale
E. Kingma, F. Dolsma, L. M. Inigo de la Cruz, L. Laan
The paper introduces a method to quantify gene disruption fitness effects using TIS data in yeast, showing that average read counts per insertion site provide a robust fitness metric.
bioRxiv · Sep 8, 2023Evolutionary Biology