L. Laan

Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP

This study investigates how Cdc42 GTPase cycling is regulated by the GEF Cdc24 and GAP Rga2 through oligomerization-dependent mechanisms and synergistic interactions.

Global genetic rewiring during compensatory evolution in the yeast polarity network

This study shows that compensatory mutations in yeast rewiring gene fitness across cellular processes, not just individual genes, highlighting the role of functional overlap and network interconnectedness.

Plasmonic Enhancement of Protein Function

This study demonstrates plasmonic enhancement of protein function in living cells by coupling designed nanoparticles to Archaerhodopsin proteins, increasing fluorescence and response speed.

Quantification of GTPase cycling rates of GTPases and GTPase : effector mixtures using GTPase Glo™ assays

The paper describes the use of GTPase Glo assays to quantify GTPase cycling rates and interactions with effector proteins.

Saturated Transposon Analysis in Yeast as a One-step Method to Quantify the Fitness Effects of Gene Disruptions on a Genome-Wide Scale

The paper introduces a method to quantify gene disruption fitness effects using TIS data in yeast, showing that average read counts per insertion site provide a robust fitness metric.

A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation

This paper provides a guide for in vitro reconstitution of Cdc42 GTPase activity and its regulation, detailing expression, purification, and assay methods.

Epigenetic feedback on noisy expression boosts evolvability

The paper models how epigenetic feedback on protein expression noise reduces fitness penalties and enhances evolvability by mitigating epistatic barriers in yeast.

Predicting an epistasis-rich genotype-phenotype map with a coarse-grained bottom-up model of budding yeast polarity

This paper develops a coarse-grained model of budding yeast polarity to predict genotype-phenotype relationships, revealing how epistasis influences evolutionary paths and fitness.

Adaptability and evolution of the cell polarization machinery in budding yeast

The paper investigates how the cell polarization machinery in budding yeast evolves and adapts by analyzing its resilience and redundancy through modeling, theory, and experiments.

Pleiotropy allows recovery of phenotypic plasticity in constant environments

This study shows that phenotypic plasticity can recover in both fluctuating and constant environments through pleiotropic interactions, challenging the idea that plasticity only evolves in fluctuating conditions.

Chromosome loss rate in cells of different ploidy can be explained by spindle self-organization

The paper develops a mathematical model to explain how chromosome loss rates vary with ploidy by considering spindle dynamics and mitotic arrest duration.

Patterns of conservation and diversification in the fungal polarization network

The paper examines the evolution of the fungal polarization network across 200 species, finding strong conservation of 16 core proteins and lineage-specific variation in others.