A General Method to Accurately Count Molecular Complexes and Determine the Degree of Labelling in Cells Using Protein Tags
S. A. Tashev, J. Euchner, K. Yserentant, S. Hänselmann, F. Hild, W. Chmielewicz, J. Hummert, F. Schwörer, N. Tsopoulidis, S. Germer, Z. Sassmannshausen, O. T. Fackler, U. Klingmüller, D.-P. Herten
This paper introduces ProDOL, a method to measure degree of labelling in cells using protein tags, and applies it to study HIV-1 Nef's effect on CD4 T cell activation.
bioRxiv · Sep 8, 2023 · PreprintCell Biology