Stacking effects on mutation detection by T4 DNA ligation within dimeric DNA origami triangle barcodes for single-molecule nanopore analysis
Dorothy Aboagye-Mensah, Samuel Confederat, Fahad Alammar, Lekshmi Kailas, Abimbola Feyisara Adedeji Olulana, Alex Stopar, Allen W. Nicholson, Neil H. Thomson, Paolo Actis, Matteo Castronovo
This paper develops a method using DNA origami barcodes and T4 DNA ligation to detect DNA point mutations with nanopore analysis, showing enhanced stacking effects that influence ligation efficiency.
bioRxiv · Jan 9, 2024 · PreprintSynthetic Biology