Paolo Actis

This paper demonstrates single-molecule nanopore sensing using a solid polymer electrolyte instead of liquid electrolytes, enhancing sensitivity and enabling robust, portable sensing platforms.

This study empirically validates a composite fractional noise model for nanopore signals across diverse experimental conditions, showing it accurately captures noise structure and separates deterministic from stochastic components.

This study benchmarks sub-50 nm quartz nanopipette emitters for native electrospray ionization mass spectrometry across multiple platforms and protein sizes, showing improved signal-to-noise and reduced adduction.

This paper presents a method using DNA origami disassembly driven by miRNA to detect small RNA, showing robust detection in RNAse-containing environments.

This study uses nanopipette electrospray ionisation ion mobility mass spectrometry to investigate how buffer conditions affect α-synuclein conformational dynamics.

This paper introduces a polymer electrolyte solid-state nanopore method to monitor endonuclease activity at physiological salt conditions, demonstrating real-time detection of restriction enzyme and CRISPR-Cas9 RNP cleavage.

This study uses nanobiopsy and next-generation sequencing to assess subcellular mtDNA mutation loads in human tissue from mitochondrial disease patients.

This paper introduces a method to profile the transcriptome of live cells over time, showing treatment induces transcriptionally stable cells in glioblastoma.

This paper develops a method using DNA origami barcodes and T4 DNA ligation to detect DNA point mutations with nanopore analysis, showing enhanced stacking effects that influence ligation efficiency.

This paper introduces a subcellular biopsy technology to isolate organelles like mitochondria from human tissue, demonstrating its ability to surpass the operational limit of laser capture microdissection.