High-throughput cell-free profiling of SARS-CoV-2 RBD variants enables rapid and quantitative in vitro affinity landscape mapping
Laura Grasemann, Jiami Han, Julia Tischler, Fatemeh Arefi, Maria Andrea Gonzales Castillo, Edward B. Irvine, Ningning Chen, Sai T. Reddy, Sebastian J. Maerkl
The paper develops a high-throughput method to profile SARS-CoV-2 RBD variants' affinity against antibodies, generating binding energy landscapes for variant analysis.
bioRxiv · Sep 23, 2025 · PreprintSystems Biology