Sebastian J. Maerkl

The paper develops a T7 RNA polymerase regulatory toolbox for cell-free systems, enabling gene circuit design and biosensing of various biomolecules with high sensitivity.

The paper investigates how transcription factor affinity, abundance, and localization affect gene expression and finds that promoter binding-site strength has the most significant impact.

The paper demonstrates that the PURE cell-free system can be reconstituted from non-ribosomal proteins synthesized by the system itself.

The paper develops a high-throughput method to profile SARS-CoV-2 RBD variants' affinity against antibodies, generating binding energy landscapes for variant analysis.

This paper designs phosphorylation-induced protein switches that control transcription in cells, demonstrating their functionality in synthetic signaling.

The paper demonstrates continuous in situ synthesis of a complete set of tRNAs in a cell-free system to support sustained protein expression.

This paper designs proteins that target neosurfaces from protein-ligand complexes using a deep learning approach, validated experimentally against three drug-bound protein complexes.

This study demonstrates a mesoscale dialysis system that extends protein synthesis in the PURE cell-free system to 12.5 days with a yield of 1.16 mg/mL GFP.

The paper improves the PURE cell-free system by reducing non-ribosomal protein concentration and increasing synthesis efficiency, enabling self-regeneration of all 36 non-ribosomal proteins.

The paper introduces C2CAplus, a one-pot isothermal DNA amplification method that improves upon C2CA by simplifying the process and increasing yield.

This paper describes a method to directly encapsulate biomolecules in semi-permeable microcapsules using double-emulsions, enabling applications in diagnostics and therapeutics.

This paper improves protein synthesis in cell-free systems using microfluidic chemostats with dialysis membranes, increasing synthesis duration and yield.

The paper identifies a robust, perfectly adapted Pho regulon state in S. cerevisiae under intermediate Pi conditions and proposes transport limitations as the mechanism for adaptation.

The paper introduces CFPU, a microfluidic device for high-throughput automated in vitro circuit characterization, enabling sustained steady-state conditions and dynamic gene circuit analysis.

This paper demonstrates molecular self-regeneration in a synthetic cell model using a minimal transcription-translation system and identifies key factors for robust system function.

The paper uses a cell-free system and microfluidic device to study synthetic transcription factor tuning mechanisms and build gene regulatory networks.

The paper introduces a low-cost method to produce the PURE cell-free system with improved protein synthesis yield.

The paper introduces a microfluidic device with pneumatic valves for precise reagent injection and mixing in emulsion drops, demonstrating its use in DNA titration experiments for green fluorescent protein synthesis.

This paper describes a microfluidic transfection device that enables high-throughput mammalian protein expression with controlled culturing and real-time imaging.

The paper develops a microfluidic biodisplay with 768 biopixels that detects arsenic in water using engineered cells and provides a visible signal for contamination.

This study shows that adding GreA and GreB to a cell-free system boosts E. coli RNA polymerase transcription rates and improves genetic network performance.