Sortase A-mediated farnesylation of Cdc42 in vitro
Sophie Tschirpke, Nynke Marije Hettema, Benjamin Spitzbarth, Mark A. R. de Geus, Frank van Opstal, Rienk Eelkema, Liedewij Laan
This paper introduces a Sortase A-based method to farnesylate Cdc42 in vitro, enabling efficient and reproducible prenylation for biochemical studies.
ACS Synthetic Biology · May 13, 2026Peer-reviewedBiochemistry