Spatially Resolved Determination of Small Molecule Protein Affinities by Hydrogen Deuterium Exchange Mass Spectrometry
De Lin, Luma G. Magalhães, Joel McMillan, T.C. Eadsforth, G. A. Stewart, Kieran R. Cartmill, Vincent L. G. Postis, Glenn Robert Masson
This paper introduces an HDX-MS workflow to estimate dissociation constants for ligand-protein interactions at peptide and amino acid resolution, enabling spatially resolved affinity mapping.
bioRxiv · Nov 13, 2025 · PreprintBiophysics