The paper develops bi-substrate inhibitors of SARS-CoV-2 nsp14 methyltransferase using structure-guided design, achieving nanomolar potency but limited cellular antiviral activity.

RSC Medicinal Chemistry · Jan 1, 2026Peer-reviewedBiochemistry

Mechanistic Insights into Dual-Active Liver and Blood-Stage Antiplasmodials

Mukul Rawat, Nonlawat Boonyalai, Cindy Smidt, Madeline R. Luth, Daisy Chen, Andrew Plater, John M. Post, De Lin, Joel McMillan, T.C. Eadsforth, Sonia Moliner Cubel, Oliver Billker, Julian C. Rayner, Francisco‐Javier Gamo, Beatriz Baragaña, , Lee M

This paper identifies targets of dual-active antimalarials by evolving resistance to nine compounds and validates them using CRISPR and molecular docking.

mBio · Nov 24, 2025Peer-reviewedMicrobiology

Spatially Resolved Determination of Small Molecule Protein Affinities by Hydrogen Deuterium Exchange Mass Spectrometry

De Lin, Luma G. Magalhães, Joel McMillan, T.C. Eadsforth, G. A. Stewart, Kieran R. Cartmill, Vincent L. G. Postis, Glenn Robert Masson

This paper introduces an HDX-MS workflow to estimate dissociation constants for ligand-protein interactions at peptide and amino acid resolution, enabling spatially resolved affinity mapping.

bioRxiv · Nov 13, 2025 · PreprintBiophysics

Characterisation of RNA guanine-7 methyltransferase (RNMT) using a small molecule approach

Lesley-Anne Pearson, Alain‐Pierre Petit, César Mendoza-Martínez, Fiona Bellany, De Lin, Sarah Niven, Rachel Swift, Thomas Eadsforth, Paul K. Fyfe, Marilyn Paul, Vincent L. G. Postis, Xiao Hu, Victoria H. Cowling, David W. Gray

This study characterizes small molecule inhibitors of the HsRNMT-RAM complex, showing competitive binding and uncompetitive inhibition of RNA cap methylation.

Biochemical Journal · Jan 27, 2025Peer-reviewedBiochemistry