Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin
T. Dau, G. Bartolomucci, J. Rappsilber
The paper evaluates protease alternatives to trypsin for protein identification in S. pombe, showing that sequential digestion with trypsin improves identification rates for several proteases.
bioRxiv · Jan 30, 2020Biochemistry