Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells
G. D. Galles, D. T. Infield, C. J. Clark, M. L. Hemshorn, S. Manikandan, F. S. Fazan, A. Rasouli, E. Tajkhorshid, J. D. Galpin, R. B. Cooley, R. A. Mehl, C. A. Ahern
This paper introduces new pyrrolysine-based aminoacyl-tRNA synthetase/tRNA pairs to site-specifically encode fluorinated phenylalanine residues in bacterial and mammalian cells, enabling experimental assessment of aromatic-pi interactions.
bioRxiv · Apr 12, 2022 · PreprintBiochemistry