CRISPR/Cas9-mediated Knock-in of an Optimized TetO Repeat for Live Cell Imaging of Endogenous Loci
I. Tasan, G. Sustackova, L. Zhang, J. Kim, M. Sivaguru, M. HamediRad, Y. Wang, J. Genova, J. Ma, A. S. Belmont, H. Zhao
The paper introduces SHACKTeR, a method for live cell imaging of specific chromosomal regions using CRISPR/Cas9 and TetO repeats, successfully labeling 9 loci in HCT116 cells with up to 20% efficiency.
bioRxiv · Jul 12, 2017Cell Biology