An ONT sequencing framework for hantavirus genome assembly, variant analysis, and defective viral genome characterization from whole-segment amplicons
Léna Vandenabeele, Frances M. Twohig, Anne Tuiskunen Bäck, Magnus Sjöblom, Rebecca Lantto, Gabriella Svala, Janne Tynell, Sandra Broddesson, Robert Dyrdak, Wanda Christ, Silke A. Riesle‐Sbarbaro, Joseph B. Prescott, Steven B. Bradfute, Daniel Pearce Depledge, Benjamin E. Nilsson-Payant
This paper introduces an Oxford Nanopore sequencing framework for hantavirus genome assembly and variant analysis, revealing defective viral genomes as a new feature of hantavirus populations.
bioRxiv · Oct 20, 2026 · PreprintMicrobiology