The paper develops a method to isolate large DNA fragments using DNA gap repair in E. coli, achieving a 203 Kbp BAC insert with improved efficiency using longer homology arms.

1 citation

E. coliDNA methylation

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Molecular BiologybioRxivPreprintJan 17, 2019

DNA Gap Repair-Mediated Site-Directed Mutagenesis is Different from Mandecki and Recombineering Approaches

George T. Lyozin, Luca Brunelli

The paper compares three site-directed mutagenesis methods in E. coli, finding that DNA gap repair is simpler and background-free compared to Mandecki and recombineering.

2 citations

E. coliexperimental assay

𝕏 PostBluesky