Author

Luca Brunelli

Children's Hospital & Medical CenterORCID 0000-0002-9542-2243Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Molecular Biology· 2

Signature Assays, Organisms & Methods

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Organism:E. coli· 2 Approach:experimental assay· 2 Assay:DNA methylation· 1 Research Focus:variant calling· 1

Frequent Co-Authors

George T. Lyozin· 2 shared

Activity by Year

2019-2019 · peak 2/yr

2 papers

Recent·Most cited

Molecular BiologybioRxivPreprintFeb 14, 2019

Isolation of Full Size BAC Inserts by DNA Gap Repair in E. coli

George T. Lyozin, Luca Brunelli

The paper develops a method to isolate large DNA fragments using DNA gap repair in E. coli, achieving a 203 Kbp BAC insert with improved efficiency using longer homology arms.

1 citation

E. coliDNA methylation

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Molecular BiologybioRxivPreprintJan 17, 2019

DNA Gap Repair-Mediated Site-Directed Mutagenesis is Different from Mandecki and Recombineering Approaches

George T. Lyozin, Luca Brunelli

The paper compares three site-directed mutagenesis methods in E. coli, finding that DNA gap repair is simpler and background-free compared to Mandecki and recombineering.

2 citations

E. coliexperimental assay

𝕏 PostBluesky