Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted PGCs
Fenghua Zhang, Xianmei Li, Mudan He, Ye Ding, Feng Xiong, Golpour Amin, Zuoyan Zhu, Yonghua Sun
This study develops a method combining CRISPR/Cas9 and PGC transplantation to efficiently generate maternal-zygotic mutants of embryonic lethal genes in zebrafish.
Journal of genetics and genomics/Journal of Genetics and Genomics · Jan 1, 2020Peer-reviewedDevelopmental Biology