RNA-seq mixology: designing realistic control experiments to compare protocols and analysis methods
Aliaksei Z Holik, Charity W Law, Ruijie Liu, Zeya Wang, Wenyi Wang, Jaeil Ahn, Marie-Liesse Asselin-Labat, Gordon K Smyth, Matthew E Ritchie
This paper introduces an RNA-seq mixture experiment using two cancer cell lines to benchmark library preparation kits and analysis methods, finding that total RNA kits and voom with quality weights perform best.
bioRxiv · Jul 19, 2016Genomics