Quantification of Cas9 binding and cleavage across diverse guide sequences maps landscapes of target engagement
E. A. Boyle, W. R. Becker, H. B. Bai, J. S. Chen, J. A. Doudna, W. J. Greenleaf
The paper quantifies Cas9 binding and cleavage across diverse guide sequences, revealing sequence-specific variations in efficacy and specificity, and develops a predictive biophysical model.
bioRxiv · Sep 9, 2020Biochemistry