Author

J. S. Chen

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4 Biophysics· 2 Immunology· 2 Bioengineering· 1 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 9 Research Focus:protein folding & dynamics· 6 Research Focus:drug response / drug discovery· 4 Research Focus:host-pathogen & immune response· 3 Organism:human· 3 Assay:DNA methylation· 2 Organism:mouse· 2 Assay:proteomics· 2

Frequent Co-Authors

J. A. Doudna· 6 shared A. Yildiz· 2 shared C. B. Wilen· 2 shared E. Ma· 2 shared J. S. Weinstein· 2 shared J. Wei· 2 shared L. B. Harrington· 2 shared M. M. Alfajaro· 2 shared

Activity by Year

2017-2024 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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6 shared papersBiochemistry

J. A. Doudna

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Author profile →

10 papers

Recent·Most cited

A novel microporous biomaterial vaccine platform for long-lasting antibody mediated immunity against viral infection.

D. P. Mayer, M. Nelson, D. Andriyanova, O. Q. Antao, J. S. Chen, P. O. Scumpia, W. M. Weaver, S. Deshayes, J. S. Weinstein

This paper develops a novel microporous biomaterial vaccine platform, VaxMAP, which enhances antibody-mediated immunity against viral infections compared to traditional vaccines.

bioRxiv · Jan 30, 2024Immunology

Pneumatic Controlled Nano-Sieve for Efficient Capture and Release of Nanoparticles

A. Nanaware, T. Kranbuhl, J. Ching, J. S. Chen, X. Chen, H. Tu, K. Du

The paper introduces a pneumatic nano-sieve device for capturing and releasing nanoparticles, demonstrating efficient capture with high flow rates and micro-grooves.

bioRxiv · Sep 30, 2022 · PreprintBioengineering

High-affinity, neutralizing antibodies to SARS-CoV-2 can be made in the absence of T follicular helper cells

J. S. Chen, R. D. Chow, E. Song, T. Mao, B. Israelow, K. Kamath, J. Bozekowski, W. A. Haynes, R. B. Filler, B. L. Menasche, J. Wei, M. M. Alfajaro, W. Song, L. Peng, L. Carter, , S. C. Eisenbarth

The paper shows that high-affinity, neutralizing antibodies against SARS-CoV-2 can be produced without T follicular helper cells through alternative pathways.

bioRxiv · Jun 10, 2021Immunology

Cyclooxgenase-2 is induced by SARS-CoV-2 infection but does not affect viral entry or replication

J. S. Chen, M. M. Alfajaro, J. Wei, R. D. Chow, R. B. Filler, S. C. Eisenbarth, C. B. Wilen

This study investigates the effect of NSAIDs on SARS-CoV-2 infection and finds that COX-2 is induced by the virus but NSAIDs do not impact viral entry or replication.

bioRxiv · Sep 24, 2020 · PreprintMicrobiology

Quantification of Cas9 binding and cleavage across diverse guide sequences maps landscapes of target engagement

E. A. Boyle, W. R. Becker, H. B. Bai, J. S. Chen, J. A. Doudna, W. J. Greenleaf

The paper quantifies Cas9 binding and cleavage across diverse guide sequences, revealing sequence-specific variations in efficacy and specificity, and develops a predictive biophysical model.

bioRxiv · Sep 9, 2020Biochemistry

Molecular mechanism of off-target effects in CRISPR-Cas9

C. G. Ricci, J. S. Chen, Y. Miao, M. Jinek, J. A. Doudna, J. A. McCammon, G. Palermo

This paper uses molecular dynamics simulations to uncover the molecular mechanism of off-target effects in CRISPR-Cas9, identifying how DNA mismatches affect HNH domain activation and cleavage.

bioRxiv · Sep 19, 2018Biophysics

CRISPR-Cas12a target binding unleashes single-stranded DNase activity

J. S. Chen, E. Ma, L. B. Harrington, X. Tian, J. A. Doudna

This study shows that CRISPR-Cas12a activates non-specific single-stranded DNA cleavage upon guide RNA-dependent DNA binding, distinguishing it from CRISPR-Cas9.

bioRxiv · Nov 29, 2017Biochemistry

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

J. S. Chen, Y. S. Dagdas, B. P. Kleinstiver, M. M. Welch, L. B. Harrington, S. H. Sternberg, J. K. Joung, A. Yildiz, J. A. Doudna

This study identifies a mechanism by which CRISPR-Cas9 variants discriminate target mismatches and introduces a hyper-accurate variant with improved specificity.

bioRxiv · Aug 12, 2017Biochemistry

A thermostable Cas9 with increased lifetime in human plasma

L. Harrington, D. Paez-Espino, J. S. Chen, E. Ma, B. T. Staahl, N. C. Kyrpides, J. A. Doudna

This paper describes a thermostable Cas9 variant from Geobacillus stearothermophilus that functions at higher temperatures and has increased stability in human plasma, expanding CRISPR-Cas9 applications.

bioRxiv · May 16, 2017Biochemistry

A Conformational Checkpoint Between DNA Binding And Cleavage By CRISPR-Cas9

Y. S. Dagdas, J. S. Chen, S. H. Sternberg, J. A. Doudna, A. Yildiz

This study identifies a conformational checkpoint in CRISPR-Cas9 that regulates DNA cleavage by requiring divalent cations and preventing cleavage at off-target sites with sequence mismatches.

bioRxiv · Mar 30, 2017Biophysics