Selection of Extended CRISPR RNAs with Enhanced Targeting and Specificity
A. Herring-Nicholas, H. Dimig, M. Roesing, E. A. Josephs
The paper presents a method to screen CRISPR gRNA variants with 5-nucleotide extensions to enhance Cas9 specificity for target sites while reducing off-target activity.
bioRxiv · Jan 11, 2023Bioengineering