Author

E. A. Josephs

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 5 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Assay:DNA methylation· 2 Research Focus:drug response / drug discovery· 2 Research Focus:perturbation response prediction· 2 Organism:plant (other)· 2 Research Focus:benchmarking· 1 Research Focus:cell type annotation· 1 Assay:CRISPR screen· 1

Frequent Co-Authors

A. Herring-Nicholas· 2 shared R. Tinker-Kulberg· 2 shared T. Islam· 2 shared T. Supakar· 2 shared A. Ligaba-Osena· 1 shared A. Sadremomtaz· 1 shared D. R. LaJeunesse· 1 shared H. Dimig· 1 shared

Activity by Year

2021-2024 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 11 mutual co-author links

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2 shared papersBioengineering

A. Herring-Nicholas

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Compartmentalized CRISPR Reactions (CCR) for High-Throughput Screening of Guide RNA Potency and Specificity

T. Supakar, A. Herring-Nicholas, E. A. Josephs

This paper introduces CCR, a method to screen gRNA potency and specificity in vitro by confining CRISPR reactions in microemulsions, enabling high-throughput validation of large gRNA variant libraries.

bioRxiv · May 7, 2024Bioengineering

Genome Editing Outcomes Reveal Mycobacterial NucS Participates in a Short-Patch Repair of DNA Mismatches

T. Islam, E. A. Josephs

This study shows that NucS in mycobacteria is involved in a short-patch DNA mismatch repair mechanism, distinct from canonical MMR, by directly repairing mismatches within 5-6 bp.

bioRxiv · Oct 23, 2023Microbiology

The "Duckweed Dip": Aquatic Spirodela polyrhiza Plants Can Efficiently Uptake Dissolved, DNA-Wrapped Carbon Nanotubes from Their Environment for Transient Gene Expression

T. Islam, S. Kalkar, R. Tinker-Kulberg, T. Ignatova, E. A. Josephs

This paper demonstrates that duckweeds can uptake DNA-wrapped carbon nanotubes from their environment for transient gene expression without requiring agrobacterium or high nanomaterial doses.

bioRxiv · Aug 21, 2023 · PreprintBioengineering

Digital data storage on DNA tape using CRISPR base editors

A. Sadremomtaz, R. F. Glass, J. E. Guerrero, D. R. LaJeunesse, E. A. Josephs, R. Zadegan

This paper introduces a DNA-based data storage system using CRISPR base editors to write and recover digital data on synthesized DNA tapes.

bioRxiv · Feb 7, 2023Bioengineering

Selection of Extended CRISPR RNAs with Enhanced Targeting and Specificity

A. Herring-Nicholas, H. Dimig, M. Roesing, E. A. Josephs

The paper presents a method to screen CRISPR gRNA variants with 5-nucleotide extensions to enhance Cas9 specificity for target sites while reducing off-target activity.

bioRxiv · Jan 11, 2023Bioengineering

Polyvalent Guide RNAs for CRISPR Antivirals

R. Bagchi, R. Tinker-Kulberg, T. Supakar, S. Chamberlain, A. Ligaba-Osena, E. A. Josephs

This paper designs polyvalent guide RNAs (pgRNAs) for CRISPR antivirals that can target multiple viral sites simultaneously, showing improved suppression of plant RNA viruses compared to monovalent gRNAs.

bioRxiv · Feb 25, 2021Bioengineering