Ultra-Fast Bioorthogonal Spin-Labeling and Distance Measurements in Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids
S. Jana, E. G. B. Evans, H. S. Jang, S. Zhang, H. Zhang, A. Rajca, S. E. Gordon, W. N. Zagotta, S. Stoll, R. A. Mehl
This paper introduces a fast bioorthogonal reaction system for in-cell spin-labeling and DEER spectroscopy, enabling structural studies of proteins in live mammalian cells.
bioRxiv · Jan 26, 2023 · PreprintBiochemistry