Author

S. E. Gordon

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5 Biophysics· 4 Physiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Research Focus:protein folding & dynamics· 5 Assay:fluorescence / live-cell microscopy· 4 Organism:human· 4 Research Focus:cell signaling & trafficking· 3 Assay:proteomics· 3 Research Focus:cell type annotation· 2 Assay:cryo-EM· 1

Frequent Co-Authors

W. N. Zagotta· 7 shared R. A. Mehl· 4 shared E. G. Evans· 3 shared J. Petersson· 3 shared S. Stoll· 3 shared A. Stratiievska· 2 shared C. M. Jones· 2 shared E. N. Senning· 2 shared

Activity by Year

2018-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 24 mutual co-author links

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7 shared papersBiophysics

W. N. Zagotta

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10 papers

Recent·Most cited

Ligand-Coupled Conformational Changes in a Cyclic Nucleotide-Gated Ion Channel Revealed by Time-Resolved Transition Metal Ion FRET

P. Eggan, S. E. Gordon, W. N. Zagotta

This study uses time-resolved tmFRET to analyze conformational changes and energetics in the SthK C-terminal region upon cAMP and cGMP binding, revealing large structural changes with cAMP and partial agonism with cGMP.

eLife · Dec 10, 2024Peer-reviewedBiophysics

Genetic code expansion, click chemistry, and light-activated PI3K reveal details of membrane protein trafficking downstream of receptor tyrosine kinases

D.-S. Koh, A. Stratiievska, S. Jana, S. C. Otto, T. M. Swanson, A. Nhim, S. Carlson, M. Raza, L. A. Naves, E. N. Senning, R. A. Mehl, S. E. Gordon

The paper uses optogenetic PI3K activation and genetic code expansion to study RTK signaling, showing PI3K alone can traffic TRPV1 and insulin receptors to the plasma membrane.

eLife · Aug 20, 2024Peer-reviewedPhysiology

Long-distance tmFRET using bipyridyl- and phenanthroline-based ligands

S. E. Gordon, E. G. Evans, S. C. Otto, M. Tessmer, K. D. Shaffer, M. T. Gordon, J. Petersson, S. Stoll, W. N. Zagotta

This paper expands the working distance of tmFRET to 50 Å using new ligands, enabling precise measurement of conformational changes in maltose binding protein under physiological conditions.

bioRxiv · Oct 9, 2023Biophysics

Measuring conformational equilibria in allosteric proteins with time-resolved tmFRET

W. N. Zagotta, E. G. Evans, P. Eggan, M. Tessmer, K. D. Shaffer, J. Petersson, S. Stoll, S. E. Gordon

This paper develops a fluorescence method to measure protein conformational dynamics and energetics using time-resolved tmFRET and validates it with EPR spectroscopy.

bioRxiv · Oct 9, 2023Biophysics

Ultra-Fast Bioorthogonal Spin-Labeling and Distance Measurements in Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids

S. Jana, E. G. B. Evans, H. S. Jang, S. Zhang, H. Zhang, A. Rajca, S. E. Gordon, W. N. Zagotta, S. Stoll, R. A. Mehl

This paper introduces a fast bioorthogonal reaction system for in-cell spin-labeling and DEER spectroscopy, enabling structural studies of proteins in live mammalian cells.

bioRxiv · Jan 26, 2023 · PreprintBiochemistry

An improved fluorescent noncanonical amino acid for measuring conformational distributions using time-resolved transition metal ion FRET

W. N. Zagotta, B. S. Sim, A. K. Nhim, M. M. Raza, E. G. Evans, Y. Venkatesh, C. M. Jones, R. A. Mehl, J. Petersson, S. E. Gordon

This paper introduces an improved fluorescent noncanonical amino acid, Acd, for time-resolved tmFRET to measure conformational distributions in proteins.

bioRxiv · May 10, 2021Biophysics

Genetic Encoding of a Highly Photostable, Long Lifetime Fluorescent Amino Acid for Imaging in Mammalian Cells

C. M. Jones, D. M. Robkis, R. J. Blizzard, M. Munari, Y. Venkatesh, T. S. Mihaila, A. J. Eddins, R. A. Mehl, W. N. Zagotta, S. E. Gordon, E. J. Petersson

The paper introduces a genetically encodable fluorescent amino acid, Acd, with superior photostability and long fluorescence lifetime for use in FLIM and protein studies.

bioRxiv · Apr 5, 2021Biochemistry

Multimerization of Homo sapiens TRPA1 ion channel cytoplasmic domains

G. Q. Martinez, S. E. Gordon

This study investigates how modifications to TRPA1 cytoplasmic domains affect channel gating by analyzing interactions between N-terminal ARDs and C-terminal coiled-coils in an in vitro system.

bioRxiv · Nov 8, 2018Biochemistry

Reciprocal regulation among TRPV1 channels and phosphoinositide 3-kinase in response to nerve growth factor

A. Stratiievska, S. Nelson, E. N. Senning, J. D. Lautz, S. E. P. Smith, S. E. Gordon

The paper shows TRPV1 channels and PI3K reciprocally regulate each other in response to NGF, with TRPV1's N-terminal ARD enhancing PI3K activity.

bioRxiv · Jun 13, 2018Biochemistry

Visualizing conformational dynamics of proteins in solution and at the cell membrane

S. E. Gordon, M. Munari, W. N. Zagotta

This paper introduces ACCuRET, a method for measuring conformational dynamics in proteins with high precision, applicable to both soluble and membrane proteins.

bioRxiv · Apr 3, 2018Biochemistry