IVT-seq reveals extreme bias in RNA-sequencing
Nicholas F Lahens, Ibrahim Halil Kavakli, Ray Zhang, Katharina Hayer, Michael B Black, Hannah Dueck, Angel Pizarro, Junhyong Kim, Rafael A Irizarry, Russell S Thomas, Gregory R Grant, John B Hogenesch
IVT-seq identifies significant technical biases in RNA-seq library preparation and sequencing, revealing coverage inconsistencies and rRNA depletion as major contributors.
Genome Biology · Jun 30, 2014Peer-reviewedGenomics