System-level studies of a cell-free transcription-translation platform for metabolic engineering
Yong Y. Wu, Hirokazu Sato, Hongjun Huang, Stephanie J. Culler, Julia Khandurina, Harish Nagarajan, Tae Hoon Yang, Stephen Van Dien, Richard M. Murray
The paper demonstrates the use of a cell-free TX-TL system to rapidly engineer and optimize the BDO pathway for improved product yield.
bioRxiv · Aug 3, 2017 · PreprintBioengineering