Triplet Quenching by Active Site Cysteine Residues Improves Photostability in Fatty Acid Photodecarboxylase
Junfeng Ma, Jason M. D. Kalapothakis, Harry J. Spacey, Linus O. Johannissen, Eugene Shrimpton-Phoenix, Muralidharan Shanmugam, Michiyo Sakuma, Christopher W. Wood, Perdita E. Barran, Derren J. Heyes, Nigel Shaun Scrutton
This study shows that adding cysteine residues to FAP improves its photostability by quenching harmful triplet states of the FAD cofactor.
bioRxiv · Oct 10, 2025 · PreprintBiochemistry