Nigel Shaun Scrutton

Biosensor-guided evolution of chalcone synthase enhances biosynthesis of natural and non-natural flavanones

The paper improves microbial production of diverse flavanones by evolving chalcone synthases using a biosensor-based screening approach.

From Discovery Proteomics to Process-Informed Monitoring in Biomanufacturing Chassis Development

The paper evaluates proteomics platforms for metabolic pathway analysis in biomanufacturing chassis organisms, showing varying proteome coverage across different instruments.

Expanding all-α-helical protein space through rational computational design

The paper designs de novo all-α-helical proteins using computational methods and validates their stability and structure.

The single nucleotide polymorphism rs1053230 modulates kynurenine 3-monooxygenase stability and is associated with cognitive and mood phenotypes

This study investigates the functional impact of SNPs rs1053230 and rs2275163 on KMO protein stability and interactions, linking rs1053230 to depressive mood symptoms.

Integrated structural dynamics uncover new modes of B12 photoreceptor activation

The paper describes the photoactivation mechanism of the B12 photoreceptor CarH using structural and spectroscopic methods, revealing unique intermediates that drive tetramer dissociation.

Triplet Quenching by Active Site Cysteine Residues Improves Photostability in Fatty Acid Photodecarboxylase

This study shows that adding cysteine residues to FAP improves its photostability by quenching harmful triplet states of the FAD cofactor.

Rapid Assessment of Size, Shape, and Chemical Complementarity of Ligands for Computational Protein Design

The paper develops a computational method to design proteins that bind small molecules tightly and specifically, validated experimentally with X-ray crystallography and functional assays.

De novo production of prenylnaringenin compounds by a metabolically engineered Escherichia coli

This paper reports the de novo production of prenylnaringenin compounds in Escherichia coli using metabolic engineering and CRISPR-Cas12a to boost DMAPP availability.

Structure-based analysis unveils co-origin of LPOR and nitrogenase-like proteins

The paper identifies structural links between nitrogenase-like proteins, LPOR, and chlorophyllide oxidoreductases, revealing their evolutionary origins and functional evolution through structural analysis.

Design and fabrication of recombinant reflectin-based Bragg reflectors: bio-design engineering and photoisomerism induced wavelength modulation

The paper designs and fabricates a bio-inspired reflectin-based Bragg reflector that modulates optical properties via visible light-induced photoisomerism.

PartsGenie: an integrated tool for optimising and sharing synthetic biology parts

PartsGenie is a tool for designing and sharing synthetic biology parts, integrating optimization, data standards, and repository access.

Selenzyme: Enzyme selection tool for pathway design

Selenzyme is an online tool that helps select enzymes for metabolic pathway design by analyzing sequence, reaction, and organism-related data.