Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention
Qiang Zhang, Hui-Li Xing, Zhi-Ping Wang, Haiyan Zhang, Fang Yang, Xuechen Wang, Qijun Chen
The paper reports high-frequency off-target mutagenesis in Arabidopsis using CRISPR/Cas9 despite high specificity scores and presents strategies to prevent it.
Plant Molecular Biology · Feb 22, 2018Peer-reviewedMolecular Biology