A De Novo Design Strategy to Convert FAcD from Dimer to Active Monomer
Ke-Wei Chen, Cuizhen Wang, Hui-si Huang, Xing Wu, Jianan Chen, Jia-He Qiu, Kin Kuan Hoi, Tianyu Sun, Jian‐bo Wang, Yun‐Dong Wu
This paper presents a de novo design strategy to convert the dimeric enzyme FAcD into an active monomer, achieving high catalytic activity with a mutant showing improved thermal stability.
bioRxiv · Jan 11, 2025 · PreprintBioengineering