Endogenous GFP tagging in the diatom Thalassiosira pseudonana
O. Nam, I. Grouneva, L. C. M. Mackinder
This paper develops a CRISPR/Cas9-based method for endogenous GFP tagging in diatoms, enabling precise and scarless knock-in of GFP at native genomic locations for accurate protein localization and abundance tracking.
bioRxiv · Sep 30, 2022 · PreprintPlant Biology