A recombineering pipeline to clone large and complex genes in Chlamydomonas
T. Emrich-Mills, G. Yates, J. Barrett, I. Grouneva, C. S. Lau, C. E. Walker, T. K. Kwok, J. W. Davey, M. P. Johnson, L. Mackinder
This paper develops a recombineering pipeline to clone large and complex genes in Chlamydomonas, achieving high success rates for genes previously difficult to clone using PCR-based methods.
bioRxiv · May 6, 2020Plant Biology