CRISPRi-Seq for the Identification and Characterisation of Essential Mycobacterial Genes and Transcriptional Units
Timothy J. de Wet, Irene Gobe, Musa M. Mhlanga, Digby F. Warner
This study introduces CRISPRi-Seq for essential gene and transcriptional unit identification in mycobacteria, showing strong overlap with Tn-Seq and functional validation of operon structure.
bioRxiv · Jun 29, 2018 · PreprintMicrobiology